Which of the following is TRUE about DNA sequencing methods?

A、Taq polymerase is quite heat sensitive;

B、Purified fragments of DNA cut from plasmid or amplified by polymerase chain reaction (PCR);

C、Clones of DNA fragments are denatured to single strands, and one of the strands is hybridized to an oligonucleotide primer;

D、New strands of DNA are synthesized from the end of the primer